Search results for " Enzymatic cleaning"

Novel proteases from marine organisms with potential interest in restoration procedure

2013

In the last decades, molecular biology allowed the development of innovative protocols in the field of conservation/restoration of cultural assets. In this work new hydrolyses, isolated from marine invertebrate organisms, are applied to remove protein layers form works of art surface. Proteolytic zymography assay evidenced that these enzymes are active in a broad temperature range, between 4 degrees and 37 degrees C. The enzymatic cleaning by these proteases, tested on wooden furniture of the second half of the eighteenth century showed positive results, without needing to heat the enzyme solution or the surface on which they were applied. The present report proposes novel proteases more ap…

MODERN TECHNOLOGIES FOR CONSERVATION OF VILLA ZITO PAINTINGS COLLECTION (PALERMO – SICILY)

2017

In this study we describe the conservative integrated approach performed on 40 paintings, dating from different period (XVII- XX century), belonging to “Fondazione Sicilia “ and exposed in Villa Zito paint gallery in Palermo (Mazzocca 2015). Through a multidisciplinary approach both constitutive materials and state of conservation have been determined. Particularly, pigments and executive techniques were identified by non-invasive diagnostic investigation (XRF analysis, UV fluorescence acquisition, and IR Reflectography) as well as previous restorations events. The study has included the revealing of microbial colonization by using non-invasive sampling methods (Nylon membrane fragment) and…

Titolo: Novel proteases from marine organisms with potential interest in restoration procedure

2012